Can you defy call the glory if you used the buff cost?

Yes, you can use Defy to counter Call to Glory, even if you chose to pay the buff cost to ignore its Energy cost.

Reasoning

1. Defy's Restriction: Defy can counter a spell that costs no more than 4 Energy and no more than 1 Power.
2. Printed vs. Paid Cost: As established by RiftJudge FAQs (#6358, #7464, and #3777), effects that check a card's cost refer to its printed cost, not any modified cost, discounted cost, or total cost paid.
3. Call to Glory's Cost: Call to Glory has a printed cost of 3 Energy and 0 Power. Because its printed Energy cost (3) is less than or equal to 4, and its printed Power cost (0) is less than or equal to 1, it is a valid target for Defy.
4. Effect of Paying the Buff Cost: When you pay the additional buff cost to ignore the Energy cost of Call to Glory, this only modifies the cost for that specific casting. It does not change the card's printed cost, which is the only value Defy uses to determine its legality as a target.

Therefore, because Call to Glory meets the printed cost requirements, it remains a legal target for Defy regardless of how it was paid for.

Note: I could not fully verify this ruling against the retrieved rules.